Development of Fluorescence-Tagged SARS-CoV-2 Virus-like Particles by a Tri-Cistronic Vector Expression System for Investigating the Cellular Entry of SARS-CoV-2Article Published on 2022-12-192023-07-11 Journal: Viruses [Category] COVID19(2023년), [키워드] ACE2 cell entry endocytosis fluorescence labeling fusion Rab5 SARS-CoV-2 virus-like particle. [DOI] 10.3390/v14122825 PMC 바로가기
An engineered 5-helix bundle derived from SARS-CoV-2 S2 pre-binds sarbecoviral spike at both serological- and endosomal-pH to inhibit virus entrySARS-CoV-2 S2에서 파생된 조작된 5나선 번들은 바이러스 진입을 억제하기 위해 혈청학적 pH와 엔도솜 pH 모두에서 사베코바이러스 스파이크를 사전 결합합니다.Article Published on 2022-12-012022-09-11 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 변종, [키워드] 5-helix bundle (5HB) acute respiratory syndrome acute respiratory syndrome coronavirus bind binding Broad-spectrum inhibition can not cell membrane construction coronavirus Evidence feature fusion heptad repeat highlighting host cell host cells IC50 infections inhibit neutralize peptide peptides pre-fusion predominant Protein sarbecovirus sarbecovirus Sarbecoviruses SARS-CoV-2 SARS-CoV-2 pseudovirus SARS-CoV-2 S2 SARS-CoV-2 variant SARS-CoV-2 variants serological severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 structural basis These data Treatment viral entry viral entry. viral spike virus entry virus infection [DOI] 10.1080/22221751.2022.2095308 PMC 바로가기 [Article Type] Article
Ganoderma microsporum immunomodulatory protein acts as a multifunctional broad-spectrum antiviral against SARS-CoV-2 by interfering virus binding to the host cells and spike-mediated cell fusionArticle Published on 2022-11-012022-11-15 Journal: Biomedicine & pharmacotherapy = Biomédecine & phar [Category] SARS, 변종, 치료제, [키워드] ACE2 ACE2-expressing cells acute respiratory syndrome alleviate Antiviral binding blocked Broad-spectrum antiviral against SARS-CoV-2 caused Cell Cell fusion cell lines cell-free virus cells characteristic coronavirus Delta determine Diseases downregulation Effect evaluated examined flow cytometry fluorescence microscopy fluorescent Fungal immunomodulatory proteins fusion GMI HEK293T cell host cell immunomodulatory immunoprecipitation in vitro Infection Infectivity ingredient inhibit inhibited inhibitor Interaction investigated less mechanism multifunctional omicron Protein pseudotyped virus reported SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 pseudovirus SARS-CoV-2 pseudovirus SARS-CoV-2 pseudovirus infection SARS-CoV-2 spike protein SARS-CoV-2 variant spike mutation spike variant spike variants Strains Viral binding. virus were used [DOI] 10.1016/j.biopha.2022.113766 PMC 바로가기
Graphene-Binding Peptide in Fusion with SARS-CoV-2 Antigen for Electrochemical Immunosensor ConstructionArticle Published on 2022-10-172022-11-15 Journal: Biosensors [Category] COVID19(2023년), SARS, 진단, 치료기술, [키워드] antibody Antibody detection Antibody titers Antigen applied caused characterized conditions construction decrease demonstrated detect diagnosing Diagnosis diagnostic DPV Efficiency electrochemical immunosensor emerging disease Fourier transform fusion Graphene human serum increase in Infection limit of detection Linear regression Microscopy peptide platform positive probe pulse Raman spectroscopy reduced response resulting S protein SARS-CoV-2 SARS-CoV-2 antibodies SBP SEM sera solid-binding peptide (SBP). tested the SARS-CoV-2 the SARS-CoV-2 virus traditional method was performed was used [DOI] 10.3390/bios12100885 PMC 바로가기
Synthesis and structure-activity relationship study of saponin-based membrane fusion inhibitors against SARS-CoV-2SARS-CoV-2에 대한 사포닌계 막융합 억제제의 합성 및 구조-활성 관계 연구Article Published on 2022-10-012022-09-11 Journal: Bioorganic chemistry [Category] COVID19(2023년), SARS, 변종, 치료제, [키워드] antiviral agent Antiviral agents antiviral efficacy Cell cell entry Cell fusion Complex molecule Complex molecules synthesis component Compound coronavirus coronavirus variants COVID-19 Critical derivative develop Effectiveness endosomal experiment fusion Glucose host cell host cell membranes inhibit inhibited inhibitor inhibitors membrane fusion protease Saponin saponins Saponins. SARS-CoV-2 SARS-CoV-2 entry SARS-CoV-2 variant SARS-CoV-2 variants Serine sugar synthesis the SARS-CoV-2 transmembrane [DOI] 10.1016/j.bioorg.2022.105985 PMC 바로가기 [Article Type] Article
Metalloprotease-Dependent S2′-Activation Promotes Cell-Cell Fusion and Syncytiation of SARS-CoV-2Article Published on 2022-09-212022-11-16 Journal: Viruses [Category] SARS, 변종, [키워드] absence ACE2 ACE2 receptor assays benefit cell-cell fusion Combination contribute contributing to COVID-19 ectodomain Factor fusion in vitro induce inhibitor metalloproteases not reduce observation patients with COVID-19 pharmacological promote reduce SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 spike protein SARS-CoV-2 variant serine protease serine proteases significantly Spike protein syncytiation. the SARS-CoV-2 the spike protein treatment strategy [DOI] 10.3390/v14102094 PMC 바로가기
Baculovirus-Free SARS-CoV-2 Virus-like Particle Production in Insect Cells for Rapid Neutralization AssessmentArticle Published on 2022-09-202022-11-15 Journal: Viruses [Category] SARS, 진단, [키워드] ACE2 Analysis antibodies antibody approach assays assessment binding candidate Cell cellular cellular assay donors enzyme evaluate expression expression plasmid expression vector flow cytometric fluorescent fusion genomic information GFP insect insect cell insect cells labeling membrane membrane protein monoclonal antibody NTA Particle plasmid transfection Production purification quantification Rapid required SARS-CoV-2 SARS-CoV-2 envelope secondary antibody sera Spike protein TEM therapeutic transmission electron microscopy vaccinated individuals virus Virus-like particle Virus-like particles (VLPs). VLP VLPs [DOI] 10.3390/v14102087 PMC 바로가기
Screening and Characterization of Shark-Derived VNARs against SARS-CoV-2 Spike RBD ProteinArticle Published on 2022-09-182022-11-16 Journal: International Journal of Molecular Sciences [Category] SARS, 변종, [키워드] ACE2 addition affinity angiotensin-converting enzyme 2 antibodies antibody fragment Antibody therapeutics Antigen bi-paratopic VNAR binding binding affinities characterization classical complexes COVID-19 Delta domain epitope exhibited FIVE flexible fusing fusion gel filtration immunized inaccessible motif omicron Protein RBD RBD binding receptor recognize reported SARS-CoV SARS-CoV-2 SARS-CoV-2 RBD Screening shark spike Spike protein the RBD the SARS-CoV-2 variants variants. VNAR wildtype [DOI] 10.3390/ijms231810904 PMC 바로가기
Impact of SARS-CoV-2 RBD Mutations on the Production of a Recombinant RBD Fusion Protein in Mammalian CellsArticle Published on 2022-08-242022-11-16 Journal: Biomolecules [Category] SARS, 변종, [키워드] accumulation assist B.1.351 B.1.351 variant cells chaperone chaperones contribute correlated COVID-19 diagnostic diagnostics disulphide bond domain E484K E484K mutation effort Endoplasmic reticulum expressed fusion Impact interfere mammalian mammalian cells misfolding mutant Mutation PDIA2 producing Production Protein protein disulphide isomerase protein yield. protocols RBD RBD mutation RBD protein RBD-Fc fusion protein recombinant recombinant RBD protein reduced resulting SARS-CoV-2 SARS-CoV-2 RBD SARS-CoV-2 variant secretion the RBD unfolded protein response UPR upregulation Vaccine variants [DOI] 10.3390/biom12091170 PMC 바로가기
Targeted escape of SARS-CoV-2 in vitro from monoclonal antibody S309, the precursor of sotrovimabArticle Published on 2022-08-242022-11-15 Journal: Frontiers in Immunology [Category] SARS, 진단, [키워드] ACE2 ACE2 binding ACE2 binding site Amino acid angiotensin-converting enzyme 2 antibody Cell cell entry class conserved domain endocytosis Entry epitope escaped Evolution Evolution of SARS-CoV-2 fusion immune Immune escape immunological in vitro infections inhibit SARS-CoV-2 Interaction monoclonal monoclonal antibody not affected pathogenicity plasticity precursor Receptor-binding domain replicated S309 SARS-CoV-2 Sotrovimab. susceptible the spike protein vaccination viral strain wild-type SARS-CoV-2 [DOI] 10.3389/fimmu.2022.966236 PMC 바로가기